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Previous appointments:

1. Postdoctoral Fellow at University of Kansas Medical Center, Kansas City, Kansas, USA (1994-1998).

2. Visiting Associate Professor at University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA (2009-2011).

Research interests:

Studies on the bacterial proteins involved in the transcription and pathogenesis


Contact:

Address: Department of Biochemistry
Centenary Campus
Bose Institute
P-1/12 C.I.T. Scheme VII-M
Kolkata - 700054, India
E-Mail: sau[at]jcbose.ac.in
Phone: +91-33-25693228

Research:

The primary objectives of our research are to study the structure, function, and stability of some bacterial proteins involved in the transcription and pathogenesis. We mostly employ biochemical, biophysical and genetic techniques to characterize our proteins of interest. Currently, we have been working to understand the (1) structure, function, stability of the immunophilins from both Escherichia coli and Staphylococcus aureus, (2) structure, function, stability of an anti-sigma factor from Staphylococcus aureus, (3) structure, function, stability of a global staphylococcal virulence regulator SarA, and (4) architecture of a bacteriophage repressor - operator DNA complex. The yielded data seem to develop tools suitable for gene expression and antibacterial drug discovery.

Publications:

  1. Biswas A, Ghosh S, Sinha D, Dutta A, Seal S, Bagchi A, Sau S (2018). Dimerization ability, denaturation mechanism, and the stability of a staphylococcal phage repressor and its two domains. Int J Biol Macromol. 124:903-914. IF 3.909
  2. Mandal S, Ghosh S, Sinha D, Seal S, Mahapa A, Polley S, Saha D, Sau K, Bagchi A, Sau S (2018) Alanine substitution mutations in the DNA binding region of a global staphylococcal virulence regulator affect its structure, function, and stability. Int J Biol Macromol. 113:1221-1232. IF: 3.909
  3. Sinha D, Mondal R, Mahapa A, Sau K, Chattopadhyaya R, Sau S (2018) A staphylococcal anti-sigma factor possesses a single-domain, carries different denaturant-sensitive regions and unfolds via two intermediates. PLoS One. 13: e0195416. IF: 3.234
  4. Mahapa A, Mandal S, Sinha D, Sau S, Sau K (2018) Determining the Roles of a Conserved α-Helix in a Global Virulence Regulator from Staphylococcus aureus. Protein J. 37:103-112. IF: 1.133
  5. Biswas A, Mandal S, Sau S (2017) Identification and characterization of a CI binding operator at a distant location in the temperate staphylococcal phage ф11. FEMS Microbiol Lett. 364(20). IF: 1.735
  6. Polley S, Seal S, Mahapa A, Jana B, Biswas A, Mandal S, Sinha D, Sau K and Sau S (2017) Identification and characterization of a cyclosporin binding cyclophillin from Staphylococcus aureus Newman. Bioinformation 13: 78-85. IF: 0.8
  7. Mandal S, Mahapa A, Biswas A, Jana B, Polley S, Sau K, Sau S (2016). A Surfactant-Induced Functional Modulation of a Global Virulence Regulator from Staphylococcus aureus. PLoS One. 11:e0151426. IF: 3.234
  8. Polley S, Chakravarty D, Chakrabarti G, Sau S (2016). Determining the roles of a conserved tyrosine residue in a Mip-like peptidyl-prolyl cis-trans isomerase. Int J Biol Macromol. 87:273-80. IF: 3.138
  9. Polley S, Chakravartya D, Chakrabarti G, Chattopadhyaya R, Sau S (2015) Proline substitutions in a Mip-like peptidyl-prolyl cis-trans isomerase severely affect its structure, stability, shape and activity. Biochimie Open. 1: 28–39.
  10. Mahapa A, Mandal S, Biswas A, Jana B, Polley S, Sau S, Sau K. (2015) Chemical and thermal unfolding of a global staphylococcal virulence regulator with a flexible C-terminal end. PLoS One. 10: e0122168. IF: 3.234
  11. Polley S, Jana B, Chakrabarti G, Sau S (2014) Inhibitor-induced conformational stabilization and structural alteration of a mip-like peptidyl prolyl cis-trans isomerase and its C-terminal domain. PLoS One. 9: e102891. IF: 3.234
  12. Biswas A, Mandal S, Sau S (2014) The N-terminal domain of the repressor of Staphylococcus aureus phage Φ11 possesses an unusual dimerization ability and DNA binding affinity. PLoS One. 9: e95012. IF: 3.234
  13. Jana B and Sau S (2012) The helix  located between the two domains of a Mip-like peptidyl prolyl cis-trans isomerase is crucial for its structure, stability and the protein folding ability. Biochemistry. 51:7930-9. IF: 3.377
  14. Bandhu A, Ganguly T, Jana B, Chakravarty A, Biswas A, and Sau S (2012) Biochemical characterization of L1 repressor mutants with altered operator DNA binding activity. Bacteriophage. 2:79-88. 
  15. Jana B, Bandhu A, Mondal R, Biswas A, Sau K, Sau S (2012) Domain Structure and Denaturation of a Dimeric Mip-like Peptidyl-Prolyl cis-trans Isomerase from Escherichia coli. Biochemistry. 51:1223-37. IF: 3.377
  16. Luong TT, Sau K, Roux C, Sau S, Dunman PM, and Lee CY (2011) S. aureus ClpC divergently regulates capsule via sae and codY in strain Newman but activates capsule via codY in strain UAMS-1 and in saeS-repaired strain Newman. J Bacteriol. 193: 686-94. IF: 3.594
  17. Bandhu A, Ganguly T, Jana B, Mondal R and Sau S (2010) Regions and residues of an asymmetric operator DNA interacting with the monomeric repressor of temperate mycobacteriophage L1. Biochemistry. 49: 4235-43. IF: 3.377
  18. Mondal R, Chanda PK, Bandhu A, Jana B, Lee CY, and Sau S (2010). Detection of the cell wall-affecting antibiotics at sublethal concentrations using a reporter Staphylococccus aureus harboring drp35 promoter – lacZ transcriptional fusion. BMB Rep. 43: 468-473. IF: 1.948
  19. Chanda PK, Bandhu A, Jana B, Mondal R, Ganguly T, Sau K, Lee CY, Chakrabarti G,  and Sau S (2010) Characterization of an unusual cold shock protein from Staphylococcus aureus. J Basic Microbiol. 50: 1-8. IF: 1.198
  20. Mondal R, Ganguly T, Chanda PK, Bandhu A, Jana B, Sau K, Lee CY, and Sau S (2010). Stabilization of the primary sigma factor of Staphylococcus aureus  by core RNA polymerase. BMB Rep 43: 176-181. IF: 1.948
  21. Ganguly T, Das M, Bandhu A, Chanda PK, Jana B, Mondal R, and Sau S (2009) Physicochemical properties and distinct DNA binding capacity of the repressor of temperate Staphylococcus aureus phage phi11. FEBS J. 276: 1975-85. IF: 4.25
  22. Majumdar T, Chattopadhyay P, Saha DR, Sau S and Mazumder S (2009). Virulence plasmid of Aeromonas hydrophila induces macrophage apoptosis and helps in developing systemic infection in mice. Microb Pathog. 46: 98-107. IF: 2.007
  23. Das M, Ganguly T, Bandhu A, Mondal R, Chanda PK, Jana B and Sau S (2009). Moderately thermostable Cro repressor of the temperate S. aureus phage ф11 possessed novel DNA-binding capacity and physicochemical properties. BMB Rep 42: 160-65. IF: 1.948
  24. Bandhu A, Ganguly T, Chanda K P, Das M, and Sau S (2009) Antagonistic effects of Na+ and Mg2+ on the structure, function and stability of repressor of temperate mycobacteriophage L1. BMB Rep 42: 293-298. IF: 1.948
  25. Chanda PK, Mondal R, Sau K, and Sau S (2009) Antibiotics, arsenate and H2O2 induce the promoter of Staphylococcus aureus cspC gene more strongly than cold. J Basic Microbiol. 49:205-11. IF: 1.198
  26. Mandal P, Datta HJ, Sau S and Mandal NC (2008) The delayed early gene G23 of temperate mycobacteriophage L1 regulates the expression of deoxyribonuclease, the product of another delayed early gene of the phage. Pol J Microbiol. 57:113-119. IF: 0.76
  27. Sau S, Chattoraj P, Ganguly T, Chanda PK, and Mandal NC (2008) Inactivation of bacterial indispensable proteins by early /delayed early proteins of bacteriophages: Implication in antibacterial drug discovery. Curr Protein Pept Sci 9: 284-90. IF: 3.512 
  28. Chattoraj P, Ganguly T, Nandy RK, and Sau S (2008). Overexpression of a delayed early gene hlg1 of temperate mycobacteriophage L1 is lethal to both M. smegmatis and E. coli. BMB Rep. 41: 363-8. IF: 1.948
  29. Chanda PK, Ganguly T, Das M, Lee CY, Luong TT and Sau S (2007) Detection of antistaphylococcal and toxic chemicals by biological assay systems developed with a reporter Staphylococcus aureus strain harboring a heat shock promoter – lacZ fusion. J Biochem Mol Biol.  40:936-43. IF: 1.948
  30. Datta HJ, Mandal P, Bhattacharya R, Das N, Sau S, and N. C. Mandal. (2007) The G23 and G25 genes of temperate mycobacteriophage L1 are essential for the transcription of its late genes. J Biochem Mol Biol.  40:156-62. IF: 1.948
  31. Das M, Ganguly T, Chattoraj P, Chanda PK, Bandhu A, Lee CY and Sau S (2007) Overexpression, purification and characterization of repressor of temperate S. aureus phage ф11. J Biochem Mol Biol  40: 740-8. IF: 1.948
  32. Sau K, Gupta SK, Sau Sau S, Mandal SC and Ghosh TC. (2007). Factors influencing the synonymous codon and amino acid usage bias in a broad-host-range Vibrio phage KVP40. J Microbiol. 45: 58-63. IF: 1.276
  33. Ganguly T, Bandhu A, Chanda PK, Chattoraj P, Das M, Mandal NC and Sau S (2007). Purification and characterization of repressor of temperate mycobacteriophage L1. Virol J. 4:64.  IF: 2.09
  34. Ganguly T, Chanda PK, Bandhu A, Chattoraj P, Das M and Sau S (2006). Effects of physical, ionic, and structural factors on the binding of repressor of mycobacteriophage L1 to its cognate operator DNA. Protein Pept Lett 13: 793-798. IF: 1.124
  35. K. Sau, S. Sau, S. C. Mandal and T. C. Ghosh. (2006). Factors influencing synonymous codon and amino acid usage biases in Mimivirus. Biosystems 85: 107-13. IF: 1.548
  36. P. Mandal, P. Chakraborty, S. Sau, and N. C. Mandal (2006). Purification and Characterization of a Deoxyriboendonuclease from Mycobacterium smegmatis. J Biochem Mol Biol  39: 140-4. IF: 1.948
  37. K. Sau, S. Sau, S. C. Mandal and T. C. Ghosh. (2005). Factors influencing the synonymous codon and amino acid usage bias in an AT-rich P. aeruginosa phage PhiKZ. Acta Biochim Biophys Sin (Shanghai) 37: 625-33. IF:2.191
  38. K. Sau, S. K. Gupta, S. Sau, and T. C. Ghosh. (2005). Synonymous codon usage bias in sixteen Staphylococcus aureus phages: Implication in phage therapy. Virus Res 113: 123-31. IF: 2.324
  39. K. Sau, S. K. Gupta, S. Sau, and T. C. Ghosh. (2005). Comparative analysis of the base composition and codon usages in fourteen mycobacteriophage genomes. J Biomol Struct Dyn. 23: 63-72. IF:1.43
  40. S.K. Gupta, T. Banerjee, S. Basak, K. Sahu, S. Sau, and T.C. Ghosh (2005).  Studies on codon usage in Thermoplasma acidophilum and its possible implications on the occurrences of lateral gene transfer. J Basic Microbiol. 45: 344-354. IF: 1.00
  41. I. Datta, S. Sau, A. Sil and N. C. Mandal. (2005). The Bacteriophage  DNA Replication Protein P Inhibits the oriC DNA- and ATP-binding Functions of the DNA Replication Initiator Protein DnaA of Escherichia coli. J Biochem Mol Biol  38: 97-103. IF: 1.948
  42. I. Datta, S. Banik-Maiti, L. Adhikari, S. Sau, N. Das, and N. C. Mandal (2005). The Mutation that makes Escherichia coli Resistant to  P Gene Mediated Host Lethality is Located within the DNA Initiator Gene dnaA of the Bacterium. J Biochem Mol Biol  38: 89-96. IF: 1.948
  43. N. C. Mandal, R. Bhattacharyya, S. Sau, and B. Chaudhuri (2004) Studies on Temperate Mycobacteriophage L1: Its Physical Map, Site of Deletion in one of its Mutant, and Organization of Early, Delayed Early and Late Genes. Perspective in Cytology & Genetics 11: 81-100. Ed. G. K. Manna & S. C. Roy; AICCG Publications, Kolkata.
  44. T. Ganguly, P. Chattoraj, M. Das, P. Chanda, N.C. Mandal, C. Y. Lee, and S. Sau (2004). A point mutation at the C-terminal half of the repressor of temperate Mycobacteriophage L1 affects its binding to the operator DNA. J Biochem Mol Biol  37: 709-714. IF: 1.948
  45. K. Sahu, S. K. Gupta, S. Sau, and T. C. Ghosh (2004). Synonymous Codon Usage Analysis of Mycobacteriophage Bxz1 and its plating bacteria M. smegmatis: identification of the Highly and Lowly Expressed Genes of Bxz1 and the possible function of its tRNA species. J Biochem Mol Biol  37: 487-92. IF: 1.948
  46. S. Sau, P. Chattoraj, T. Ganguly, C. Y. Lee, and N. C. Mandal (2004). Cloning and Sequencing Analysis of the Repressor Gene of Temperate Mycobacteriophage L1. J Biochem Mol Biol  37: 254 -259. IF: 1.948
  47. C. Chattopadhyay, S. Sau, and N. C. Mandal. (2003). Cloning and Characterization of the Promoters of Temperate Mycobacteriophage L1. J Biochem Mol Biol  36: 586 - 592. IF: 1.948
  48. L. Thanh, S. Sau, Gomez M, J. C. Lee and C. Y. Lee (2002). Regulation of Staphylococcus aureus Capsular Polysaccharide Expression by agr and sarA. Infect Immun 70: 440 -50. IF: 3.933
  49. J.L. Snodgrass, N. Mohammad, J.M. Ross, S. Sau, Chia. Y. Lee and M.S. Smeltzer (1999). Functional analysis of the S.aureus collagen adhesin B domain. Infect Immun. 67:3952-9. IF: 3.933
  50. Ouyang Shu, S. Sau and Chia Y. Lee (1999). Promoter analysis of the cap8 operon involved in type 8 capsular polysaccharide production in Staphylococcus aureus. J Bacteriol, 181: 2492 –2500. IF: 3.712
  51. P. Gilpsey, Chia Y. Lee , S. Sau and M.S. Smeltzer (1998). Factors Affecting the Collagen Binding Capacity of Staphylococcus aureus. Infect Immun, 66: 3170-3178. IF: 4.034
  52. S. Sau., Navneet Bhasin, Elisabeth R.Wann, J. C. Lee, T. J. Foster and  C. Y. Lee (1997). The Staphylococcus aureus  Allelic Genetic Loci for  Serotype 5 and 8 Capsule Expression Contain the Type-specific Genes Flanked by Common Genes. Microbiology, 143: 2395-2405. IF: 2.307
  53. S. Sau, Jiwen Sun and Chia Y. Lee (1997). Molecular Characterization and Transcriptional Analysis of Type 8 Capsule Genes in S. aureus. J Bacteriol, 179: 1614-1621. IF: 3.639
  54. S. Sau and Chia Y. Lee (1996). Cloning of Type 8 Capsule Genes and Analyses of Gene Clusters for the Production of Different Capsular Polysaccharides in Staphylococcus aureus. J Bacteriol, 178: 2118-2126. IF: 3.889
  55. B. Chaudhuri, S. Sau, H. J. Datta and N.C. Mandal (1993). Isolation, Characterization and Mapping of Temperature Sensitive Mutations in the Genes Essential for Lysogenic and Lytic growth of the   Mycobacteriophage L1. Virology, 194: 166-172.
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Recognition:

    Teaching:

    The following courses were taught:

    1. Molecular Microbiology

    2. Radiochemical Methods

    3. Radiological Safety

    Students:

    Image Name Designation Department Campus Contact number Email
    profile image Priya Rai Junior Research Fellow Biochemistry Centenary 25693238
    profile image Tushar Chakraborty Junior Research Fellow Biochemistry Centenary 25693235

    Former:

    Present members:

    1. Mr. Debabrata Sinha (SRF)
    2. Mr. Soham Seal (SRF)
    3. Ms. Priya Rai (JRF)
    4. Mr. Tushar Chakraborty (JRF)

    Past members:

    1. Dr. Tridib Ganguly (2007)
    2. Dr. Partho Chattoraj (2008)
    3. Dr. Malabika Das (2008)
    4. Dr. Palas K Chanda (2009)
    5. Dr. Amitava Bandhu (2012)
    6. Dr. Biswanath Jana (2013)
    7. Dr. Rajkrishna Mondal (2015)
    8. Mr. Sudip K Mitra (2016)
    9. Dr. Anindya Biswas (2017)
    10. Dr. Soumitra Polley (2018)
    11. Mr. Sukhendu Mondal  (2018)



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